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|Title: ||Investigation of yeast Grown in SSF Dring Biothanol Production from Lignocellusosic Material|
|Authors: ||Babapour, Ayda Barid|
Gavitar, Maryam Nadalipour
|Department: ||Högskolan i Borås/Ingenjörshögskolan (IH)|
|Issue Date: ||5-Jun-2012|
|Series/Report no.: ||Magisteruppsats|
|Programme: ||MSc in Resource Recovery - Industrial Biotechnology|
|Publisher: ||University of Borås/School of Engineering|
|Media type: ||text|
|Abstract: ||Ethanol produced from lignocellulosic biomass has the potential to become a promising
alternative to gasoline. In this work the simultaneous saccharification and fermentation (SSF)
technology was applied for ethanol production from hardwood with focus on cell growth,
ethanol production and contamination.
The SSF was performed at PH 5.5 and 35°C for different suspended solid concentrations
(8%, 10% and 12%) of pretreated birch slurry which contained 16 % total suspended solids.
Two different hexose fermenting yeast strain (Ethanol Red) and pentose fermenting yeast
strain were used.
Quantifying the concentration of chemical components and metabolites in the fermentation
medium demonstrated that glucose and xylose are the major fermentable sugars in the slurry.
The higher load of slurry (12%) represents a higher content of carbohydrates and potentially
higher end concentration of ethanol. Moreover, more lactic acid is produced with the lower
load of slurry (8 % or 10 %), presumably due to a result of a less inhibitory environment for
bacterial growth. In this context, acetic acid sticks out as the most important inhibitor with
concentrations of 15.2 and 12.5 and 9.7 g/l respectively in the 12 %, 10 % and 8 % (of
suspended solids) trials. Using pentose fermenting yeast may lead to higher ethanol
production, lower xylose uptake and lower lactic acid formation. Cell viability and cell
vitality determination from fermentation media in all the trails represented a sharply
decreasing trend during the fermentation for both Ethanol Red yeast strain and the pentose
fermenting strain yeast strain apparently due to cell decomposition.|
|Appears in Collections:||Magisteruppsatser (IH)|
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