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Please use this identifier to cite or link to this item: http://hdl.handle.net/2320/11322

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Title: Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
Authors: Westman, Johan O.
Taherzadeh, Mohammad J.
Franzen, Carl Johan
Department: University of Borås. School of Engineering
Other
Issue Date: 2012
Journal Title: Electronic Journal of Biotechnology
ISSN: 0717-3458
Volume: 15
Issue: 3
Pages: 1-14
Publisher: Pontificia Universidad Catolica de Valparaiso
Media type: text
Publication type: article, peer reviewed scientific
Keywords: biofuel
furfural
lignocellulose
phenolic inhibitors
Saccharomyces cerevisiae
Subject Category: Subject categories::Engineering and Technology::Industrial Biotechnology
Research Group: Biotechnology
Area of Research: Resource Recovery
Strategic Research Area: Resource Recovery
Abstract: Background: Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS8066. Results: The flocculating strain CCUG53310 could rapidly ferment all hexoses in dilute acid spruce hydrolysate, while CBS8066 was strongly inhibited in this medium. In synthetic inhibitory media, CCUG53310 was more tolerant to carboxylic acids and furan aldehydes, but more sensitive than CBS8066 to phenolic compounds. Despite the higher tolerance, the increase in expression of the YAP1, ATR1 and FLR1 genes, known to confer resistance to lignocellulose-derived inhibitors, was generally smaller in CCUG53310 than in CBS8066 in inhibitory media. The flocculation of CCUG53310 was linked to the expression of FLO8, FLO10 and one or more of FLO1, FLO5 or FLO9. Flocculation depended on cell wall proteins and Ca2+ ions, but was almost unaffected by other compounds and pH values typical for lignocellulosic media. Conclusions: S. cerevisiae CCUG53310 can be characterised as being very robust, with great potential for industrial fermentation of lignocellulosic hydrolysates relatively low in phenolic inhibitors.
DOI: 10.2225/vol15-issue3-fulltext-8
URI: http://hdl.handle.net/2320/11322
Sustainable development: sustainable development
Appears in Collections:Artiklar och rapporter / Articles and reports (IH)

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