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Title: Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors
Authors: Westman, Johan O.
Babikondu, Ramesh Babu
Franzén, Carl Johan
Taherzadeh, Mohammad J
Department: University of Borås. School of Engineering
Other
Issue Date: 19-Sep-2012
Journal Title: International Journal of Molecular Sciences
ISSN: 1422-0067
Volume: 13
Issue: 9
Pages: 11881-11894
Publisher: MDPI
Media type: text
Publication type: article, peer reviewed scientific
Keywords: lignocellulosic hydrolysate
ethanol
furfural
HMF (5-hydroxymethyl furfural)
carboxylic acids
encapsulation
Saccharomyces cerevisiae
biofuel
inhibitors
q-PCR
Subject Category: Subject categories::Engineering and Technology::Industrial Biotechnology::Other Industrial Biotechnology
Research Group: Biotechnology
Area of Research: Resource Recovery
Strategic Research Area: Resource Recovery
Abstract: The ability of macroencapsulated Saccharomyces cerevisiae CBS8066 to withstand readily and not readily in situ convertible lignocellulose-derived inhibitors was investigated in anaerobic batch cultivations. It was shown that encapsulation increased the tolerance against readily convertible furan aldehyde inhibitors and to dilute acid spruce hydrolysate, but not to organic acid inhibitors that cannot be metabolized anaerobically. Gene expression analysis showed that the protective effect arising from the encapsulation is evident also on the transcriptome level, as the expression of the stress-related genes YAP1, ATR1 and FLR1 was induced upon encapsulation. The transcript levels were increased due to encapsulation already in the medium without added inhibitors, indicating that the cells sensed low stress level arising from the encapsulation itself. We present a model, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.
DOI: 10.3390/ijms130911881
URI: http://hdl.handle.net/2320/11425
Sustainable development: sustainable development
Appears in Collections:Artiklar och rapporter / Articles and reports (IH)

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