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Title: Proteomic Analysis of the Increased Stress Tolerance of Saccharomyces cerevisiae Encapsulated in Liquid Core Alginate-Chitosan Capsules
Authors: Westman, Johan O.
Taherzadeh, Mohammad J
Franzen, Carl Johan
Department: University of Borås. School of Engineering
Other
Issue Date: 2012
Journal Title: PLOS One
ISSN: 1932-6203
Volume: 7
Issue: 11
Pages: 12
Publisher: Public Library of Science
Media type: text
Publication type: article, peer reviewed scientific
Subject Category: Subject categories::Natural Sciences::Biological Sciences
Research Group: Biotechnology
Area of Research: Resource Recovery
Strategic Research Area: Resource Recovery
Abstract: Saccharomyces cerevisiae CBS8066 encapsulated in semi-permeable alginate or alginate-chitosan liquid core capsules have been shown to have an enhanced tolerance towards complex dilute-acid lignocellulose hydrolysates and the lignocellulose-derived inhibitor furfural, as well as towards high temperatures. The underlying molecular reasons for these effects have however not been elucidated. In this study we have investigated the response of the encapsulation on the proteome level in the yeast cells, in comparison with cells grown freely in suspension under otherwise similar conditions. The proteomic analysis was performed on whole cell protein extracts using nLC-MS/MS with TMT® labelling and 2-D DIGE. 842 and 52 proteins were identified using each method, respectively. The abundances of 213 proteins were significantly different between encapsulated and suspended cells, with good correlation between the fold change ratios obtained by the two methods for proteins identified in both. Encapsulation of the yeast caused an up-regulation of glucose-repressed proteins and of both general and starvation-specific stress responses, such as the trehalose biosynthesis pathway, and down-regulation of proteins linked to growth and protein synthesis. The encapsulation leads to a lack of nutrients for cells close to the core of the capsule due to mass transfer limitations. The triggering of the stress response may be beneficial for the cells in certain conditions, for example leading to the increased tolerance towards high temperatures and certain inhibitors.
DOI: 10.1371/journal.pone.0049335
URI/URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0049335
URI: http://hdl.handle.net/2320/11530
Sustainable development: sustainable development
Appears in Collections:Artiklar och rapporter / Articles and reports (IH)

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