The goal of this project is extraction of chitosan optimally by surveys of temperature changes along with 1% Sulfuric acid utilization. Microbial chitosan is isolated as a bio-component from cell wall of two kinds of Zygomycetes by some extraction methods. This project compares ability of two type Fungi (R. pulusilus and M.indicus) from Zygomycets for production of chitosan. To extract of chitosan is a combinational method with 40 %( w/w) Hydroxyl sodium for cell disruption and diluted Sulfuric acid (1% w/v) for chitosan extraction from cell wall as major chemical components. 40% NaOH is used to get different degrees of deacetylation (DD) from chitin for chitosan. In addition, it is examined 1% Sulfuric acid in a combination of temperature factor changes. It is needed dialysis for chitosan purification from bonded phosphate groups. Standard curves of acetic acid experiences for DD and water phosphate determination were accomplished. It has resulted if degree of deacetylated chitin is about 50%; it has an average lost more than 50% in 1% (v/v) Sulfuric acid, hence less recovery as a no privilege that it can be relapsed by acetone in chitosan solution. Factor of temperate in same times shows important effect on extraction yield of chitosan by 1% Sulfuric acid. Extracted chitosan in 120℃ has DD about 50%. Absolutely, its solubility will be more and it needs to an intricate solution for separation of chitosan from phosphate bonds as a major impurity by dialysis, but in 90℃, DD of chitosan is more with less solubility in water. Between two Fungi, in experienced temperatures, hence, R. pulusilus has more recovery about 0.87/AIM (g/g) in 90℃, which have more much DD than 50%, and M.indicus has 0.79/AIM (g/g) in 120℃ that it has DD about 50%.