A codigestion process was evaluated when mixing different ratios of agro-industrial residues, i.e. slaughterhouse waste (SB); manure (M); various crop residues (VC); and municipal solid waste (MSW). Methane yield (YCH4) and specific methanogenic activity (SMA) were determined by anaerobic batch digestion of the different substrates and substrate mixtures. The performance of the codigestion was also evaluated by kinetics (k0) based on the methane production rate. The investigated residues and their mixtures were found to have high potentials for the production of biogas. The codigestion process showed to be a feasible option with positive influence in both energy production and environmental care. Taking into account significance differences, the best results were obtained with a mixture ratio of 1:3:4:0.5 based on the w/w content of SB, M, VC, and MSW, respectively, which showed a proper combination of high values in YCH4, SMA and k0 during the anaerobic process. The effect of temperature suggested that thermophilic conditions are suitable to treat these residues. Response variables were severely affected by mesophilic conditions, diminishing in at least 45% of the thermophilic value when a mixture ratio of 2:3:3:0 based on the w/w content of SB:M:VC:MSW was selected. It is suggested to study the influence of individual component within the mixture for every response variable. Consecutive feedings are also suggested in order to step forward to continuous process and evaluate possible inhibitions.